Study of gene expression and transcript structure
through sequencing with Oxford Nanopore technology®.
Transcriptomics enables studying the functional dynamics of the cell through the analysis of RNA molecules expressed in a given biological condition.
While traditional methods fragment RNA losing information about the complete structure of transcripts, Oxford Nanopore technology enables full-length transcript sequencing, preserving information about the isoform structure.
Through the passage of native RNA or cDNA molecules full-length via nanopore, Oxford Nanopore measures current variations with characteristic features associated with the base sequence, enabling identification of:
With Direct RNA Sequencing it is also possible to sequence native RNA directly, avoiding retrotranscription and PCR amplification and reducing amplification bias.
Transcriptomic analysis is performed on the Oxford Nanopore Technologies® platform, enabling sequencing of full-length transcripts and analysis of isoform structure without the need for short-read reconstruction.
Learn more on the dedicated Nanopore Sequencing → page.
Sequencing of complete transcripts for isoform characterization, alternative splicing events and gene fusions.
Direct sequencing of native RNA without retrotranscription or PCR amplification.
We offer a fully end-to-end service tailored to research needs.
Our team supports the client from experimental design consultation through to biological interpretation of results.
The service includes:
The standard bioinformatics pipeline includes:
If requested, we also offer multiomics integration, upload of raw data to public repositories and support for biological interpretation of results.
Epigenomic analysis enables studying the mechanisms of gene expression regulation and DNA modifications across diverse biological contexts.
Study of expression variations of genes in diverse biological conditions.
Analysis of isoform diversity generated by splicing events.
Study of transcriptional programs that regulate development and cell differentiation.
Analysis of gene expression networks and associated molecular pathways.
Multidisciplinary expertise in genomics, bioinformatics and molecular biology.
Full support from experimental planning through to delivery of results.
Oxford Nanopore platform for whole-genome long-read sequencing.
We collaborate with academic groups, clinics and industry to adapt the workflow to each research need.
Probiomics supports transcriptomics research projects through complete sequencing
and gene expression analysis workflows, from experimental planning to biological interpretation of results.
Tell us briefly about your project or analytical need.
Our scientific team will evaluate your request and get back to you.
Tell us briefly about your project or analytical need.
Our scientific team will evaluate your request and get back to you.
